INULINASE PURIFICATION PDF

Keywords: Characterization; Inulinase; Production; Purification; Streptomyces. 1. Introduction. Inulin is a polyfructan in plant consisting of linear chains. Aspergillus niger exo-inulinase purification by three phase partitioning. Inulinase (2, 1-β-D-fructan fructanohydrolase, EC ) hydrolyses inulin into. 42(4), August , pp. Purification and characterization of Inulinase from marine bacterium,. Bacillus cereus MU S Meenakshi1, S Umayaparvathi .

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Academic Press, London, pp: Effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men. Elution was purificatiin out by 0. The analysis of the deduced inulinase amino acid sequence revealed the presence of a putative gram-positive signal peptide and a cleavage site following amino acid 39 was predicted with the program designed by Nielsen et al.

The most potent isolate was identified as Streptomyces grisenus. Inulinase was purified wit a recovery of 2. Hydrolysis of inulin from jerusalem artichake by inulinase immobilized on aminoethyl cellulose enzyme.

Elution profile of the enzyme produced streptomyces grisenus after sephadex G filtration. Present findings are in agreement with Lim et al. The fermentation products from these groups of bacteria give rise to local and systemic health effects.

After separation, the proteins were transferred to a polyvinylidene difluoride membrane, and after staining with ponceau S solution, the inulinase protein band was cut from the membrane and subjected to amino acid sequence analysis. Inulinase can be found in plants and microorganisms. Factors affecting purified inulinase enzyme: These authors concluded that inulin induces inulinase production, where as cultivation using starch, maltose and lactose as carbon source s restrains the production of this enzyme by optimizing culture conditions for maximum inulinase production, purification of streptomyces grisenius crude inulinase in cell free extracts was the aim of further research in order to increase its specific enzyme activity.

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Analysis of purified enzyme using gel electrophoresis revealed an apparent molecular weight of KDa.

Production, Purification, Characterization and Applications of Fungal Inulinases

The gel permeation chromatography on Sephadex G column provided large additional increase in the purity of the enzyme according to Ettalibi and Baratti Purification and characterizationof an endoinulinase from Xanthomonas oryzae No. Use of dinitrosalicylic acid reagent for determination of reducing sugar. In order to determine the N-terminal amino acid sequence, the protein was extracted from streptomyces grisenus cells by 8 M urea treatment and then separated in an SDS-PAGE gel as already described Laemmli, Dietary fiber, inulin and oligofructose: The results in Table 2 revealed that the maximum activity was observed at pH 7.

Oligosaccharides are compounds with great potential of use in food industry particularly interesting are fructooligosaccharids FOSbecause of their favorable functional properties such as low calorie and noncariogenic sweeteners, improvement of the intestinal microbial flora, relief of constipation, decrease of total cholesterol and lipid in the serum and promotion of animal growth Vandamme and Derycke, How to cite this article: Effect of temperature on the inulinase activity of Streptomyces grisenus.

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Microbial enzyme inulinase EC 3. Results represented in Table 8 Indicated that the inulinase activity increased with increasing the substrate concentration until 1 mu inulianse the activity was constant between 1- muinulin. However pure enzyme was stable in the presence of CaCl 2. Effect of metal ions on the purified inulinase produced by Streptomyces grisenius.

Isolation and characlerization of bacterial strains with inulinase activity. Microbial inulinases formentation process, properties and applications.

Production, Purification, Characterization and Applications of Fungal Inulinases | BenthamScience

As shown in Table 1 the maximum activity was found on putification 2nd day of incubation period. The purified inulinase enzyme was subjected to N-terminal sequence analysis. Expression, purification and characterization of an exo-B-D-Fructosidase of Streptomyces mutans.

Cite this article as: The effect of carbon source on inulinase production was shown in Table 4.